N-Cadherin anticorps (Cytoplasmic Domain)

N° du produit ABIN6252207

Cet anticorps Souris Monoclonal détecte spécifiquement N-Cadherin dans WB, IF et FACS. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour N-Cadherin anticorps (Cytoplasmic Domain) (ABIN6252207)

Antigène: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp N-Cadherin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 13A9

Détail du produit anti-N-Cadherin anticorps

Épitope: Cytoplasmic Domain

Purification: Protein G affinity chromatography

Immunogène: A human partial recombinant protein corresponding to the CDH2/NCAD cytoplasmic domain was used as the immunogen for this N-Cadherin antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/10^6 cells in 0.1ml

IF: 1-2 μg/mL

WB: 0.5-1 μg/mL

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the N-Cadherin antibody to be titered up or down for optimal performance.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur N-Cadherin

Antigène: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Autre désignation: N-Cadherin

Sujet: Recognizes a protein of ~140  kDa, identified as N-Cadherin (NCAD), also known as CDH2 and CD325. NCAD is a member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. NCAD/CD325 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, the protein has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis. Furthermore, it is normally involved in inducing cell cycle arrest and its expression is frequently deregulated in cancer cells. Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin.

ID gène: 1000

UniProt: P19022

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane